Cryogenic preservation of embryos ofProchilodus lineatus(Valenciennes, 1836) (Characiforme; Prochilodontidae)
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منابع مشابه
Cryogenic preservation of embryos of Prochilodus lineatus (Valenciennes, 1836) (Characiforme; Prochilodontidae).
SummaryWhile the freezing techniques of mammal embryos have been providing promising results, the cryopreservation of teleostean eggs and embryos have remained unsuccessful up to now. Therefore, this work aimed to develop a procedure of cryogenic preservation of embryos of Prochilodus lineatus and to observe, at both structural and ultrastructural levels, the morphological alterations that took...
متن کاملStructural and ultrastructural analysis of embryonic development of Prochilodus lineatus (Valenciennes, 1836) (Characiforme; Prochilodontidae).
This survey was performed to characterize the embryogenesis of Prochilodus lineatus. Seven stages of embryo development were identified--zygote, cleavage, blastula, gastrula, segmentation, larval and hatching--after a period of incubation of 22 h (24 degrees C) or 14 h (28 degrees C). The following cleavage pattern was identified: the first plane was vertical (2 blastomeres); the second was ver...
متن کاملStructural and ultrastructural characteristics of the yolk syncytial layer in Prochilodus lineatus (Valenciennes, 1836) (Teleostei; Prochilodontidae).
The yolk syncytial layer (YSL) has been regarded as one of the main obstacles for a successful cryopreservation of fish embryos. The purpose of this study was to identify and characterize the YSL in Prochilodus lineatus, a fish species found in southeastern Brazil and considered a very important fishery resource. Embryos were obtained through artificial breeding by hormonal induction. After fer...
متن کاملDiel and seasonal movements of grumatã Prochilodus lineatus (Valenciennes 1836) (Characiformes: Prochilodontidae) in the Sinos River, Southern Brazil.
Prochilodus lineatus is a reophilic migratory species of economical importance for local fisheries which is widely distributed in Brazil. The present study investigated diel and seasonal movement patterns, spawning migration and habitat use of P. lineatus in the Sinos River, Southern Brazil. Between August 2002 and March 2004, 19 grumatãs were tagged internally with digitally coded radio transm...
متن کاملTransformation in Escherichia coli: cryogenic preservation of competent cells.
Escherichia coli cells prepared for transformation by treatment with cold 0.1 M CaCl2 remained viable and competent after storage at -82 degrees C in 15% glycerol; thawed-cell samples yielded up to 10(6) transformants per microgram of plasmid deoxyribonucleic acid.
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ژورنال
عنوان ژورنال: Zygote
سال: 2009
ISSN: 0967-1994,1469-8730
DOI: 10.1017/s0967199408004991